Other

Part:BBa_K277032:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-20)


3L.3_23.B1.06


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 712
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
    Illegal AgeI site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed using overlap extension PCR protocol described in RFC38


Source

Synthetic Yeast

References